Adefunke Iyabo AKINOLA2024-06-112024-06-112023-12M.Schttps://repository.lcu.edu.ng/handle/123456789/508Respiratory Syncytial virus (RSV), an enveloped, non-segmented RNA, is one of the most common causes of severe respiratory infections in children and older adults worldwide. This study investigated RSV and Influenza Viruses A and B in older adults and children aged 5 and below who tested negative for COVID-19. A total number of 206 COVID-19 negative samples were tested, viral RNA was extracted using a QUIGEN RNA Purification kit and Real time PCR was performed using an Allplex TM RSV/Flu A/Flu B kit. Results showed a mean age of 2.97 for children and 33.50 for adults. There was a negative association between gender and infections with RSV (P-value 0.589) and influenza B (p-value 0.250) both ≥ 0.05 in children, but a positive association in adults. (p-value, 0.000 ≤ 0.005). Approximately, 52.0% of the total participants were from Oyo South while 37% from Oyo central and 5% from Oyo North, 6% did not disclose their location. The prevalence of influenza B and RSV in children 2.8% each was in Oyo Central, while in adults, the prevalence of influenza A and B, 1% each was in Oyo Central and Oyo South, and 2.0%; RSV, 1. % from Central and 1% from those who did not disclose their location. There was no positive association between location and outcome of infection with RSV and Influenza B (p- value 0.482). The prevalence of RSV in children was 2.8%, Influenza A was 0% and Influenza B was 2.8%, while in older adults, RSV was 2% and influenza A and B were 1% each. This study detected RSV, influenza A and B viral infections in COVID-19-negative individuals. There is a need to investigate other possible etiologies of respiratory tract infections in Sars -cov-2-negative symptomatic individuals. Keywords: Prevalence, infections, RSV, RNA, Influenza. Word Count: 293enPrevalenceinfectionsRSVRNAInfluenza.Thesis