Browsing by Author "OYEKOLA Wasiu Bode"

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    In vitro Phytochemical and Antibacterial Evaluation of Phoenix dactylifera L. Fruit and Seed Extracts against Beta-lactamase Producing Clinical Isolates Associated with Urinary Tract Infections
    (Lead City University, Ibadan, 2024-12) OYEKOLA Wasiu Bode
    Urinary tract infection (UTIs) cause by beta-lactamase producing bacteria is challenging to treat due to antibiotic resistance and high cost of drugs. The study evaluated the phytochemical and antibacterial activity of Phoenix dactylifera L. fruit and seed extracts against beta-lactamase-producing bacteria isolated from urinary tract infections. Phytochemical screening of the extracts revealed the presence of terpenoids, flavonoids, phenols, alkaloids, tannins, and saponins. The methanol extract of the fruit contained the highest concentrations, with phenol, alkaloid, and tannin 208.8±9.95mg/ml, 174.5±1.08mg/ml, and 142.2±1.03mg/ml contents. Flavonoid and saponin contents were also substantial in methanol extracts, 94.46±1.04mg/ml and 128.1±13.2mg/ml respectively. Extracts were tested at concentrations of 150, 100, 75, and 60 mg/mL using the agar well diffusion method, and zones of inhibition were measured. Results showed that the methanolic extracts exhibited the most significant antibacterial activity among the tested solvents. For fruit extracts, methanolic fractions showed inhibition zones of 16.0±1.41 mm at 150 mg/ml against Klebsiella pneumoniae, 16.0±1.41 mm against Escherichia coli, and 19.5±7.78 mm against Enterobacter cloacae. In contrast, ethyl acetate demonstrated moderate activity against E. coli (17.0±1.41 mm, and Pseudomonas luteola mm. Chloroform and aqueous extracts showed little to no inhibition across all isolates. Seed extracts showed similar trends, with methanolic extracts producing inhibition zones of 15.5±0.70 mm at 150 mg/ml against K. pneumoniae, 14.5±2.12 mm against E. coli and 18.0±1.41 mm against P. luteola. Ethyl acetate demonstrated comparable activity against E. coli (15.0±1.41 mm, and P. luteola (16.5±3.53 mm. Synergistic effects were observed with combinations of fruit and seed extracts, where methanolic combinations produced zones of 22.0±1.41 mm at 150 mg/ml against K. pneumoniae and 19.0±1.41 mm against P. luteola. Ethyl acetate combinations were also effective, with 19.5±2.12 mm inhibition against E. coli. Methanolic extracts of P. dactylifera fruit and seeds showed potent antibacterial activity, particularly against K. pneumoniae, E. coli, and P. luteola, suggesting their potential as alternative treatments for infections caused by beta-lactamase-producing bacteria. Keywords:Phoenix dactylifera Linn, antibacterial, beta-lactamase, urinary tract infections, synergistic effect. Word count:300