Browsing by Author "Suliat Adigun MUSTAPHA"

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    Identification and Molecular Characterization of Fungi Associated with Rotting Solanum lycopersicum Fruits Obtained from Certain Markets in Oyo and Osun State, Nigeria
    (Lead City University, 2022-12) Suliat Adigun MUSTAPHA
    Solanum lycopersicum is the second most important crop after potato across the globe. This important crop is however highly perishable and subjected to rot due to the effect of microorganism. Fungi has been described as the most destructive phytopathogens of Solanum lycopersicum which can appear symptomless on the crop and they have been attributed to 41% loss in Nigeria. The molecular characterization of fungi associated with Solanum lycopersicum fruits rot was studied in certain markets of Oyo and Osun state using two varieties namely; Royal and Cherry (Cerasiforme)and the objective of this study was to detect and identify molecular characterization of fungi responsible for Solanum lycopersicum fruits rot. Thirty-two rotting Solanum lycopersicum fruits were assigned to four groups with 8 samples in each group. Direct culture plate method was used to isolate fungi and the gDNA was analyzed using PCR, Sanger sequencing method and result was obtained using Blast on NCBI data base. The result from the Blast identified 6 different fungi from both varieties and the four locations. Geotrichum candidum and Rhizopus delemar were isolated from Eleekara market of Oyo state from both varieties with frequency of 4 out the 4 samples and a percentage of 18.2%. Aspergillus flavus and Pichia kudriavzevii were identified from Sasa market of Oyo state with the frequency of 4 out 4 samples and a percentage of 18.2%. Aspergillus niger and Penicillium citrinum were identified from Oluwo market of Osun state with frequency of 3 out of 4 samples and percentage of 13.6% for both. Special preventive methods like resistant cultivar, basic sanitary rules, sun drying and organic preservative to minimize Solanum lycopersicum fruits rots caused by fungal organism is very paramount. The storage of tomato should be done at a temperature and relative humidity that does not favour the growth of fungi. Keywords: Solanum lycopersicum, Blast, gDNA, PCR, Fungi Word Count: 297