Phytochemical determination and in vitro assessment of antibacterial activities of Delonix regia (Bojer ex Hook) against some bacteria associated with urinary tract infection
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2022-12
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Lead City University
Abstract
Antibiotic resistance and the side effects of synthetic medicines have led to more people using plant materials to treat a wide range of illnesses. In this study, the antibacterial activity of leaves and bark of Delonix regia (Bojer ex Hook). The content's qualitative and quantitative aspects were evaluated. The effects of leaf and bark extracts of this plant on human pathogens such as Escherichia coli, Proteus mirabilis, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter cloacae, and Acinetobacter nosocomialis were examined in vitro with methanol, ethanol, hexane, and distilled water. Agar well diffusion technique was employed throughout the experimentation. It was found that the ethanolic extracts of the leaves had the most inhibitory impact on the test microorganisms of all the extractants. The Minimum Inhibitory Concentration (MIC) of each extract was determined. Concentrations of 59.16 mg/ml, 39.33 mg/ml, 29.5 mg/ml, and 23.6 mg/ml, both sample ethanol extracts were effective against all tested bacterial isolates, except for Staphylococcus aureus and Pseudomonas aeruginosa at doses of 39.41mg/ml and 59.16mg/ml, respectively, neither the ethanol nor the methanol extracts of the leaf nor the bark were reactive against any of the test microorganisms. Ciprofloxacin (20mm), Pefloxacin (20mm), Rocephin (14mm), all suppressed Proteus mirabilis (12mm) The qualitative screening of both the leaf and the bark showed substantial presence of photochemical samples. With the quantitative showing tannins (0.48) having the highest concentration in n-hexane and terpenoids (0.458). Bark was 86% effective against all isolates except P.mirabilis and A.nosocomialis, whereas leaf was 20% effective against S. aureus and P. aeruginosa, making it bacteriostatic (75%). Phytochemical screening helps uncover bioactive molecules that may be used to make successful drugs. This study will aid in the development of quality, purity, and sample standards for the Delonix regia plant, which was discovered to have a number of phytochemicals with antibacterial properties.
Keywords: Delonix regia, Antibiotic Resistance, Antimicrobial Activity, Phytochemical Screening
Word Count: 300 words
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Kate Turabian