Detection and Characterization of Non-albicans Candida inClinical Isolates in Some Healthcare Facilities in Ibadan, Oyo State, Nigeria
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2022-12
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Lead City University
Abstract
Candida species are causes of nosocomial fungal infections and are among the leading causes of hospital-acquired infections. Candida infections ranging from superficial infections to highly invasive Candidiasis are increasingly being associated with nosocomial infections. Bloodstream infections resulting from Candida pathogenic species have high mortality rates. It is considered a public health menace globally. Patient samples were collected and phenotypic identification of C.auris and other non-candida albicans was done by culture on Sabouraud Dextrose agar supplemented with Chloramphenical. Novel ChromagarTM Candida Plus was also used. GPI protein-encoding genes of the isolates were detected using PCR techniques and primers sequence specific for C. auris. A total of 90 Candida species were isolated from Adeoyo Maternity Teaching Hospital 42 (46.7%), Ring Road State Hospital 45 (50%) and University College Hospital 3 (3.3%). High vaginal swabs were 39 (43%), Pus/wound swab 31 (34.4%), Ear swabs 11 (12%), Sputum, Endocervical swabs and Urine 3 (3.3%) respectively. This report showed 38.9% (n=35) C.auris. Candida species detected were C. krusei, C.famata, C.glabrata and C.tropicalis. A wound swab had a mixed growth of C.tropicalis, C.famata and C.krusei. Resistance to fluconazole was detected in all of the isolates with minimum inhibitory concentration of 16μg/mL and 0.03μg/mL, making it a total resistance in this study. There is need for further analysis on the genes responsible for the resistance. Molecular analysis showed 27C.auris with 5.8S rDNA having a 134(bp).
Keywords: Candida species, Non- albican candida, C.auris, Resistance, Polymerase chain reaction (PCR), Minimum inhibitory concentration (MIC)
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Kate Turabian