Toxicological Assessment on Clarias gariepinus (Cat Fish) Exposed to Leachates from Awotan Landfill, Ibadan Oyo State, Nigeria

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dc.contributor.authorJohn Opeoluwa OTITOOLA
dc.date.accessioned2024-07-23T14:18:09Z
dc.date.available2024-07-23T14:18:09Z
dc.date.issued2023-12
dc.description.abstractSolid waste disposal is a major environmental and public health concern in Nigeria. Leachate samples collected from Awotan landfill in Ibadan were investigated for their genotoxicity using juveniles of Clarias gariepinus as the bioindicator species. The leachates' physico-chemical qualities and heavy metal characteristics were analyzed using standard methods. Samples of juveniles were obtained from the Department of Aquaculture and Fisheries Management University of Ibadan and transported to the Biology Laboratory at Lead City University, where they were acclimatized in chlorine-free borehole water for 14 days. After the acclimation period, the toxicity assessment setup was initiated at the Animal House, Faculty of Natural and Applied Sciences. Five treatment bowls including control (0%), 5%, 10%, 25% and 50% (v/v leachate/distilled water) of leachate samples in triplicates were prepared, and 15 juveniles of Clarias gariepinus were introduced into each of the treatment bowls, where they were cultured and observed for 21 days. Haematology analysis of the blood, histopathology analyses of the liver and gel electrophoresis on p53gene were performed on C. gariepinus at the end of the culture period. The physico-chemical characteristics of the leachate (electrical conductivity, 4200 ± 13.0 S/m; total dissolved solids, 2450 ± 14.4 mg/L, nitrate, 87.00 ± 5.7 mg/L, and phosphate, 3.134 ± 0.11 mg/L) and heavy metals concentrations (copper, 9.28 ± 3.2 μm; manganese 45.64 ± 6.3 mg/L; and iron, 604.48 ± 10.62 mg/L) exceeded the SON and WHO recommended limits for physico-chemical and heavy metals. The haematological test showed that high concentrations of the leachate samples significantly reduced the number of red blood cells, haemoglobin, white blood cells, and packed cell volume in C. gariepinus. Histological examinations showed liver damage, with the highest leachate concentration (50%) leading to steatosis. The molecular analysis revealed that Clarias gariepinus, exhibits no genetic diversity within the tumour protein (TP53). Keywords: Leachates, Clarias gariepinus, Genotoxicity, Heavy metals World count: 299
dc.identifier.citationKate Turabian
dc.identifier.otherM.Sc
dc.identifier.urihttps://repository.lcu.edu.ng/handle/123456789/660
dc.language.isoen
dc.publisherLead City University
dc.relation.ispartofseriesM.Sc
dc.subjectLeachates
dc.subjectClarias gariepinus
dc.subjectGenotoxicity
dc.subjectHeavy metals
dc.titleToxicological Assessment on Clarias gariepinus (Cat Fish) Exposed to Leachates from Awotan Landfill, Ibadan Oyo State, Nigeria
dc.typeThesis

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