In-vitro Phytochemical and Antimicrobial Activity of Selected Parts of Securidaca longipendunculata Extracts on Clinical Wound Isolates from Diabetic Foot Ulcers
| dc.contributor.author | AYOOLA, Olubunmi Afolake | |
| dc.date.accessioned | 2025-07-01T12:17:28Z | |
| dc.date.available | 2025-07-01T12:17:28Z | |
| dc.date.issued | 2024-12 | |
| dc.description.abstract | Plant derived compound offer an alternative additional potential source of new antimicrobial, Securidaca longipedunculata is one such medicinal plant with a long history of use in Nigeria and African. Diabetic foot ulcers (DFUs) are a significant complication of diabetes mellitus, often leading to chronic infections due to antibiotic-resistant. This study aimed at investigating the in-vitro phytochemical and antimicrobial activities of ethanol, n- hexane, chloroform, and aqueous extracts from selected parts of S. longipendunculata (root bark, stem bark, and leaves) against wound isolates from DFUs. The tested microorganisms included Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterobacter cloacae, Streptococcus pyogenes, Acinetobacter baumannii and Candida albicans. The In-vitro phytochemical and antimicrobial activities of the plant extract were determined by agar well diffusion method and phytochemical analysis of the plant extract was carried out using standard method. The phytochemical analysis results of the extracts shown the presence of alkaloids, flavonoids, saponins, tannins, and terpenoid. The In-vitro phytochemical and antimicrobial activities results of the extracts using different solvent shown that at 150 mg/mL ethanolic extract had highest zone of inhibition 22.50± 0.5mm against (E. coli), 21.00mm ±1.40mm against (K. pneumonia) followed by S. longipendun cu lata chloroform extract at 18 ± 0.5 mm (A. baumannii) and aqueous extract haslowest value of 0.6 ± 0.0 mm (K. pneumonia). The result of ethanolic extract were compared with standard antibiotic of Amikacin ≥ 20mm and Ceftaxidime ≥ 21 mm, revealed that ethanolic extracts had a greater inhibition zone of 22.5 ± 0.5 mm and 21.0 mm ±1.40mm against E. coli and K. pneumonia respectively. Also, the ethanolic had greater zone of inhibition of 22 mm against C. albicans when compared with standard antifungi(fluconazole). The study concludes that S. longipendunculata had antimicrobial activity which can be used to develop new therapies for managing diabetic foot ulcers Keywords: Securidaca longipedunculata, Antimicrobial Activity, Phytochemical Analysis. Word count: 300 | |
| dc.identifier.citation | Kate Turabia | |
| dc.identifier.other | M.Sc | |
| dc.identifier.uri | https://repository.lcu.edu.ng/handle/123456789/1029 | |
| dc.language.iso | en | |
| dc.publisher | Lead City University, Ibadan | |
| dc.relation.ispartofseries | M.Sc | |
| dc.subject | Securidaca longipedunculata | |
| dc.subject | Antimicrobial Activity | |
| dc.subject | Phytochemical Analysis. | |
| dc.title | In-vitro Phytochemical and Antimicrobial Activity of Selected Parts of Securidaca longipendunculata Extracts on Clinical Wound Isolates from Diabetic Foot Ulcers | |
| dc.type | Thesis |