Department of Biological Sciences

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Browsing Department of Biological Sciences by Author "Abiye Oluwatosin ADENIJI"

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    Identification of Pathogenic and Multidrug Resistant Bacteria in Some Selected Fruits from Major Fruit Markets in Ibadan
    (Lead City University, 2022-12) Abiye Oluwatosin ADENIJI
    Multidrug resistance has been a great concern to human health. The occurrence of multidrug resistance among fruit pathogens has unfortunately increased during recent year. Hawking of whole fruit that has been divided into portions (fractional fruits) is very common in Ibadan metropolis and this practise exposes fruits to more microbial colonization. Also, to keep the fruits from spoilage, they are subjected to different preservative treatment, thereby subjecting the microbes on the fruit to challenging environment that may elucidate acquisition of new genes. Eighteen fruit samples of pawpaw(6), watermelon(6) and pineapple(6) hawked in fractions were purchased in order to enumerate mesophilic bacteria in them using standard methods. Heavy metal analysis was also carried out on the fruit using dry digestion method. The isolated bacteria were identified by their colonial, morphological and biochemical characteristics. Pathogens among these bacteria isolates were selected using based on their haemolysis on blood agar medium and the antibiotic suspectibility of the pathogens were determined by kirby-Bauer disk diffusion method on Muller Hinton agar medium. Heavy metal in all fruit samples was less than permitted limit and within the reference range with the exception of Lead which is greater than reference range. Based on methods used, thirty six bacteria strains were obtained and identified as Staphylococcus cerus, Streptococcus spp., Bacillus substilis Pseudomonas spp, Salmonella spp, Bacillus cerus Hemolysis test 14 strains to be pathogenic, eight of them where resistant common antibiotics used. However two strains of Bacillus cereus were highly multidrug resistant among all the strains obtained. Bacillus cereus is a food borne pathogen, capable of secreting toxins in human system, this result calls for serious public health concern. Keywords:Fruits, Haemolysis, Antibiotic Resistance, Fractionated Fruits, Ibadan, Bacillus cereus Word Count: 280