Department of Biological Sciences

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Browsing Department of Biological Sciences by Author "Bukola Marufat ADEYEMI EKEOLU"

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    Mesophilic Bacterial Isolates in Sludge and Waste water from Food Canteens in Tertiary Educational Institutions within Ibadan
    (Lead City University, 2022-12) Bukola Marufat ADEYEMI EKEOLU
    Microbiological safety of the environment around food canteens is of great importance as such environments could be potential sources of food-borne illnesses of epidemic proportions. Food canteens in and around tertiary educational institutions get a lot of patronage and this leads to the generation of a lot of waste water and development of sludge around their environments. The aim of this study is to determine the microbial profile of sludge and waste water generated by ``food canteens in selected tertiary institutions within Ibadan metropolis.36 samples of waste water, sludge and food were collected from three canteens in four tertiary educational institutions. Microbial isolation was carried out using Salmonella-Shigella, Eosine-methylene blue, Mannitol-Salt and Nutrient agars. Incubation was carried out for 24 hours at 37°C. Isolates obtained were subjected to biochemical characterization and molecular identification. Out of the 20 isolates obtained and identified, five were Pseudomonas aeruginosa, three Escherichia coli, three Proteus mirabilis, two Pectobacterium carotovorum, one Acinetobacter baumanni, two Bacillus species, one Enterobacter cloacae, one Providencia vermicola and one Enterococcus faecium. The result obtained shows that the waste water and sludge around the canteens as well as some of the foods sold contain potential pathogens. This appears to be from different potential sources which include raw food materials and fecal contamination. It is therefore of utmost importance to introduce treatment of wastewater to food canteen owners and also proper disposal method so that sludge formation around the canteens is reduced. This will help to reduce the risk of food borne illnesses among people patronising the canteens and subsequent spread to the entire community. Keywords: Food canteen, tertiary institutions, sludge, waste water, Ibadan Word count: 263 words